Coding

Part:BBa_K4121030

Designed by: Ziyu Li   Group: iGEM22_NEFU_China   (2022-10-05)


amino acid leucine（leu）coding sequence

Saccharomyces cerevisiae is commonly used as a model organism for genetic modification, and yeast amino acid deficient medium is a necessary medium for yeast transformation and hybridization. According to the types of defective amino acids, the medium can be divided into four categories, which are one-deficiency medium, two-deficiency medium, three-deficiency medium and four-deficiency medium. Leucine（leu）as one of defective amino acids is a screening marker for four-deficient Saccharomyces cerevisiae. The yeast with this sequence could grow on SD medium lacking leu.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 454
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 454
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 454
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 454
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 454
    Illegal AgeI site found at 841
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None